Original Article
Genetic variants identified by target next-generation sequencing in heart transplant patients with dilated cardiomyopathy
Variantes genéticas utilizando sequenciação de nova geração em doentes submetidos a transplante cardíaco
Elisabete Martinsa,b,d,
, Alexandra Sousaa,c,e, Paulo Canedob, Sérgio Leitea, Roberto Pintod, Manuel Campeloa,c,d, Sandra Amorimc,d, Brenda Mouraf, José Manuel Lopesb,g,h, José Carlos Machadoa,b, José Silva Cardosoa,c,d, on behalf of the FATIMA investigators
Autor para correspondência
a Department of Medicine, Faculty of Medicine, University of Porto, Portugal
b Institute for Innovation and Health Research (I3S), Porto, Portugal
c Center for Research in Health Technologies and Services (Cintesis), Porto, Portugal
d Department of Cardiology, Centro Hospitalar Universitário de São João, E.P.E., Porto, Portugal
e Department of Cardiology, Santa Maria Maior Hospital, Barcelos, Portugal
f Hospital das Forças Armadas, Porto, Portugal
g Department of Pathology, Centro Hospitalar Universitário de São João, E.P.E., Porto, Portugal
h Departament de Pathology and Oncology, Faculty of Medicine, Porto, Portugal
Lido
4657
Vezesque se leu este artigo
1604
Total PDF
3053
Total HTML
Compartilhar estatísticas
array:26 [ "pii" => "S0870255117304298" "issn" => "08702551" "doi" => "10.1016/j.repc.2019.02.006" "estado" => "S300" "fechaPublicacion" => "2019-06-01" "aid" => "1402" "copyright" => "Sociedade Portuguesa de Cardiologia" "copyrightAnyo" => "2019" "documento" => "article" "crossmark" => 1 "licencia" => "http://creativecommons.org/licenses/by-nc-nd/4.0/" "subdocumento" => "fla" "cita" => "Rev Port Cardiol. 2019;38:441-7" "abierto" => array:3 [ "ES" => true "ES2" => true "LATM" => true ] "gratuito" => true "lecturas" => array:2 [ "total" => 721 "formatos" => array:3 [ "EPUB" => 52 "HTML" => 522 "PDF" => 147 ] ] "Traduccion" => array:1 [ "en" => array:20 [ "pii" => "S217420491930176X" "issn" => "21742049" "doi" => "10.1016/j.repce.2019.07.002" "estado" => "S300" "fechaPublicacion" => "2019-06-01" "aid" => "1406" "copyright" => "Sociedade Portuguesa de Cardiologia" "documento" => "simple-article" "crossmark" => 1 "licencia" => "http://creativecommons.org/licenses/by-nc-nd/4.0/" "subdocumento" => "dis" "cita" => "Rev Port Cardiol. 2019;38:449-50" "abierto" => array:3 [ "ES" => true "ES2" => true "LATM" => true ] "gratuito" => true "lecturas" => array:2 [ "total" => 309 "formatos" => array:3 [ "EPUB" => 39 "HTML" => 193 "PDF" => 77 ] ] "en" => array:10 [ "idiomaDefecto" => true "cabecera" => "<span class="elsevierStyleTextfn">Editorial comment</span>" "titulo" => "Genomic characterization in dilated cardiomyopathy" "tienePdf" => "en" "tieneTextoCompleto" => "en" "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "449" "paginaFinal" => "450" ] ] "titulosAlternativos" => array:1 [ "pt" => array:1 [ "titulo" => "Caracterização genómica na cardiomiopatia dilatada" ] ] "contieneTextoCompleto" => array:1 [ "en" => true ] "contienePdf" => array:1 [ "en" => true ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "Marina C. Costa" "autores" => array:1 [ 0 => array:2 [ "nombre" => "Marina C." "apellidos" => "Costa" ] ] ] ] ] "idiomaDefecto" => "en" "Traduccion" => array:1 [ "en" => array:9 [ "pii" => "S0870255117304298" "doi" => "10.1016/j.repc.2019.02.006" "estado" => "S300" "subdocumento" => "" "abierto" => array:3 [ "ES" => true "ES2" => true "LATM" => true ] "gratuito" => true "lecturas" => array:1 [ "total" => 0 ] "idiomaDefecto" => "en" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0870255117304298?idApp=UINPBA00004E" ] ] "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S217420491930176X?idApp=UINPBA00004E" "url" => "/21742049/0000003800000006/v1_201909140841/S217420491930176X/v1_201909140841/en/main.assets" ] ] "itemSiguiente" => array:19 [ "pii" => "S0870255119304287" "issn" => "08702551" "doi" => "10.1016/j.repc.2019.07.002" "estado" => "S300" "fechaPublicacion" => "2019-06-01" "aid" => "1406" "copyright" => "Sociedade Portuguesa de Cardiologia" "documento" => "simple-article" "crossmark" => 1 "licencia" => "http://creativecommons.org/licenses/by-nc-nd/4.0/" "subdocumento" => "dis" "cita" => "Rev Port Cardiol. 2019;38:449-50" "abierto" => array:3 [ "ES" => true "ES2" => true "LATM" => true ] "gratuito" => true "lecturas" => array:2 [ "total" => 587 "formatos" => array:3 [ "EPUB" => 43 "HTML" => 395 "PDF" => 149 ] ] "en" => array:10 [ "idiomaDefecto" => true "cabecera" => "<span class="elsevierStyleTextfn">Editorial comment</span>" "titulo" => "Genomic characterization in dilated cardiomyopathy" "tienePdf" => "en" "tieneTextoCompleto" => "en" "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "449" "paginaFinal" => "450" ] ] "titulosAlternativos" => array:1 [ "pt" => array:1 [ "titulo" => "Caracterização genómica na cardiomiopatia dilatada" ] ] "contieneTextoCompleto" => array:1 [ "en" => true ] "contienePdf" => array:1 [ "en" => true ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "Marina C. Costa" "autores" => array:1 [ 0 => array:2 [ "nombre" => "Marina C." "apellidos" => "Costa" ] ] ] ] ] "idiomaDefecto" => "en" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0870255119304287?idApp=UINPBA00004E" "url" => "/08702551/0000003800000006/v3_201911281004/S0870255119304287/v3_201911281004/en/main.assets" ] "itemAnterior" => array:20 [ "pii" => "S0870255119304305" "issn" => "08702551" "doi" => "10.1016/j.repc.2019.07.004" "estado" => "S300" "fechaPublicacion" => "2019-06-01" "aid" => "1409" "copyright" => "Sociedade Portuguesa de Cardiologia" "documento" => "simple-article" "crossmark" => 1 "licencia" => "http://creativecommons.org/licenses/by-nc-nd/4.0/" "subdocumento" => "dis" "cita" => "Rev Port Cardiol. 2019;38:439-40" "abierto" => array:3 [ "ES" => true "ES2" => true "LATM" => true ] "gratuito" => true "lecturas" => array:2 [ "total" => 1142 "formatos" => array:3 [ "EPUB" => 61 "HTML" => 925 "PDF" => 156 ] ] "en" => array:10 [ "idiomaDefecto" => true "cabecera" => "<span class="elsevierStyleTextfn">Editorial comment</span>" "titulo" => "Control of risk factors in hypertensive patients: A task to fulfill" "tienePdf" => "en" "tieneTextoCompleto" => "en" "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "439" "paginaFinal" => "440" ] ] "titulosAlternativos" => array:1 [ "pt" => array:1 [ "titulo" => "Controlo dos fatores de risco nos doentes hipertensos – uma tarefa a cumprir" ] ] "contieneTextoCompleto" => array:1 [ "en" => true ] "contienePdf" => array:1 [ "en" => true ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "Manuela Fiuza" "autores" => array:1 [ 0 => array:2 [ "nombre" => "Manuela" "apellidos" => "Fiuza" ] ] ] ] ] "idiomaDefecto" => "en" "Traduccion" => array:1 [ "en" => array:9 [ "pii" => "S2174204919301783" "doi" => "10.1016/j.repce.2019.07.003" "estado" => "S300" "subdocumento" => "" "abierto" => array:3 [ "ES" => true "ES2" => true "LATM" => true ] "gratuito" => true "lecturas" => array:1 [ "total" => 0 ] "idiomaDefecto" => "en" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S2174204919301783?idApp=UINPBA00004E" ] ] "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0870255119304305?idApp=UINPBA00004E" "url" => "/08702551/0000003800000006/v3_201911281004/S0870255119304305/v3_201911281004/en/main.assets" ] "asociados" => array:1 [ 0 => array:19 [ "pii" => "S0870255119304287" "issn" => "08702551" "doi" => "10.1016/j.repc.2019.07.002" "estado" => "S300" "fechaPublicacion" => "2019-06-01" "aid" => "1406" "copyright" => "Sociedade Portuguesa de Cardiologia" "documento" => "simple-article" "crossmark" => 1 "licencia" => "http://creativecommons.org/licenses/by-nc-nd/4.0/" "subdocumento" => "dis" "cita" => "Rev Port Cardiol. 2019;38:449-50" "abierto" => array:3 [ "ES" => true "ES2" => true "LATM" => true ] "gratuito" => true "lecturas" => array:2 [ "total" => 587 "formatos" => array:3 [ "EPUB" => 43 "HTML" => 395 "PDF" => 149 ] ] "en" => array:10 [ "idiomaDefecto" => true "cabecera" => "<span class="elsevierStyleTextfn">Editorial comment</span>" "titulo" => "Genomic characterization in dilated cardiomyopathy" "tienePdf" => "en" "tieneTextoCompleto" => "en" "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "449" "paginaFinal" => "450" ] ] "titulosAlternativos" => array:1 [ "pt" => array:1 [ "titulo" => "Caracterização genómica na cardiomiopatia dilatada" ] ] "contieneTextoCompleto" => array:1 [ "en" => true ] "contienePdf" => array:1 [ "en" => true ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "Marina C. Costa" "autores" => array:1 [ 0 => array:2 [ "nombre" => "Marina C." "apellidos" => "Costa" ] ] ] ] ] "idiomaDefecto" => "en" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0870255119304287?idApp=UINPBA00004E" "url" => "/08702551/0000003800000006/v3_201911281004/S0870255119304287/v3_201911281004/en/main.assets" ] ] "en" => array:20 [ "idiomaDefecto" => true "cabecera" => "<span class="elsevierStyleTextfn">Original Article</span>" "titulo" => "Genetic variants identified by target next-generation sequencing in heart transplant patients with dilated cardiomyopathy" "tieneTextoCompleto" => true "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "441" "paginaFinal" => "447" ] ] "autores" => array:1 [ 0 => array:4 [ "autoresLista" => "Elisabete Martins, Alexandra Sousa, Paulo Canedo, Sérgio Leite, Roberto Pinto, Manuel Campelo, Sandra Amorim, Brenda Moura, José Manuel Lopes, José Carlos Machado, José Silva Cardoso" "autores" => array:12 [ 0 => array:4 [ "nombre" => "Elisabete" "apellidos" => "Martins" "email" => array:1 [ 0 => "elisabetemartins09@gmail.com" ] "referencia" => array:4 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0005" ] 1 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">b</span>" "identificador" => "aff0010" ] 2 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">d</span>" "identificador" => "aff0020" ] 3 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">*</span>" "identificador" => "cor0005" ] ] ] 1 => array:3 [ "nombre" => "Alexandra" "apellidos" => "Sousa" "referencia" => array:3 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0005" ] 1 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">c</span>" "identificador" => "aff0015" ] 2 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">e</span>" "identificador" => "aff0025" ] ] ] 2 => array:3 [ "nombre" => "Paulo" "apellidos" => "Canedo" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">b</span>" "identificador" => "aff0010" ] ] ] 3 => array:3 [ "nombre" => "Sérgio" "apellidos" => "Leite" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0005" ] ] ] 4 => array:3 [ "nombre" => "Roberto" "apellidos" => "Pinto" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">d</span>" "identificador" => "aff0020" ] ] ] 5 => array:3 [ "nombre" => "Manuel" "apellidos" => "Campelo" "referencia" => array:3 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0005" ] 1 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">c</span>" "identificador" => "aff0015" ] 2 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">d</span>" "identificador" => "aff0020" ] ] ] 6 => array:3 [ "nombre" => "Sandra" "apellidos" => "Amorim" "referencia" => array:2 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">c</span>" "identificador" => "aff0015" ] 1 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">d</span>" "identificador" => "aff0020" ] ] ] 7 => array:3 [ "nombre" => "Brenda" "apellidos" => "Moura" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">f</span>" "identificador" => "aff0030" ] ] ] 8 => array:3 [ "nombre" => "José Manuel" "apellidos" => "Lopes" "referencia" => array:3 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">b</span>" "identificador" => "aff0010" ] 1 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">g</span>" "identificador" => "aff0035" ] 2 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">h</span>" "identificador" => "aff0040" ] ] ] 9 => array:3 [ "nombre" => "José Carlos" "apellidos" => "Machado" "referencia" => array:2 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0005" ] 1 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">b</span>" "identificador" => "aff0010" ] ] ] 10 => array:3 [ "nombre" => "José" "apellidos" => "Silva Cardoso" "referencia" => array:3 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0005" ] 1 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">c</span>" "identificador" => "aff0015" ] 2 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">d</span>" "identificador" => "aff0020" ] ] ] 11 => array:1 [ "colaborador" => "on behalf of the FATIMA investigators" ] ] "afiliaciones" => array:8 [ 0 => array:3 [ "entidad" => "Department of Medicine, Faculty of Medicine, University of Porto, Portugal" "etiqueta" => "a" "identificador" => "aff0005" ] 1 => array:3 [ "entidad" => "Institute for Innovation and Health Research (I3S), Porto, Portugal" "etiqueta" => "b" "identificador" => "aff0010" ] 2 => array:3 [ "entidad" => "Center for Research in Health Technologies and Services (Cintesis), Porto, Portugal" "etiqueta" => "c" "identificador" => "aff0015" ] 3 => array:3 [ "entidad" => "Department of Cardiology, Centro Hospitalar Universitário de São João, E.P.E., Porto, Portugal" "etiqueta" => "d" "identificador" => "aff0020" ] 4 => array:3 [ "entidad" => "Department of Cardiology, Santa Maria Maior Hospital, Barcelos, Portugal" "etiqueta" => "e" "identificador" => "aff0025" ] 5 => array:3 [ "entidad" => "Hospital das Forças Armadas, Porto, Portugal" "etiqueta" => "f" "identificador" => "aff0030" ] 6 => array:3 [ "entidad" => "Department of Pathology, Centro Hospitalar Universitário de São João, E.P.E., Porto, Portugal" "etiqueta" => "g" "identificador" => "aff0035" ] 7 => array:3 [ "entidad" => "Departament de Pathology and Oncology, Faculty of Medicine, Porto, Portugal" "etiqueta" => "h" "identificador" => "aff0040" ] ] "correspondencia" => array:1 [ 0 => array:3 [ "identificador" => "cor0005" "etiqueta" => "⁎" "correspondencia" => "Corresponding author." ] ] ] ] "titulosAlternativos" => array:1 [ "pt" => array:1 [ "titulo" => "Variantes genéticas utilizando sequenciação de nova geração em doentes submetidos a transplante cardíaco" ] ] "resumenGrafico" => array:2 [ "original" => 0 "multimedia" => array:7 [ "identificador" => "fig0005" "etiqueta" => "Figure 1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr1.jpeg" "Alto" => 369 "Ancho" => 1405 "Tamanyo" => 105451 ] ] "descripcion" => array:1 [ "en" => "<p id="spar0035" class="elsevierStyleSimplePara elsevierViewall">Patient 3: baseline 12-lead electrocardiogram and 24-hour Holter monitoring showing left ventricular hypertrophy (A) and runs of nonsustained ventricular tachycardia (B).</p>" ] ] ] "textoCompleto" => "<span class="elsevierStyleSections"><span id="sec0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0055">Introduction</span><p id="par0005" class="elsevierStylePara elsevierViewall">Dilated cardiomyopathy (DCM) is a myocardial disease characterized by left ventricular (LV) dilatation and systolic dysfunction.<a class="elsevierStyleCrossRef" href="#bib0100"><span class="elsevierStyleSup">1</span></a> In a subgroup of DCM patients, the disease progresses to a terminal stage, requiring heart transplantation (HT), despite current pharmacological and device therapies. This unfavorable progression may be due to various factors, such as an aggressive initial toxic insult, initiation of therapy late in the course of the disease, or, especially, the presence of an adverse genetic background or of harmful genetic interactions with environmental factors. Based on family screening studies, the percentage of inheritable disease (causality attributed to genetic factors) is estimated to be over 30-50% in so-called idiopathic cases.<a class="elsevierStyleCrossRef" href="#bib0105"><span class="elsevierStyleSup">2</span></a> Examples of deleterious variants associated with sudden cardiac death or evolution to terminal DCM have been particularly reported in the <span class="elsevierStyleItalic">LMNA</span> gene.<a class="elsevierStyleCrossRef" href="#bib0110"><span class="elsevierStyleSup">3</span></a> Bearing these issues in mind, it is important to explore the molecular mechanisms involved in failure to respond to therapy, knowledge of which is necessary to develop further alternative therapeutic strategies in heart failure syndromes. Until now, assessment of DCM genetics has been hampered by genetic heterogeneity between families, with more than 50 different genes associated with DCM<a class="elsevierStyleCrossRef" href="#bib0115"><span class="elsevierStyleSup">4</span></a> and with most of the known genes, except titin, accounting for fewer than 10% of cases.<a class="elsevierStyleCrossRef" href="#bib0120"><span class="elsevierStyleSup">5</span></a> The recent development of next-generation sequencing (NGS) techniques has enabled identification of a large and growing number of genetic variants in DCM patients,<a class="elsevierStyleCrossRef" href="#bib0125"><span class="elsevierStyleSup">6</span></a> but the major challenge with this technology is the establishment of causal relationships between identified genetic variants and cardiomyopathy phenotypes.<a class="elsevierStyleCrossRef" href="#bib0130"><span class="elsevierStyleSup">7</span></a> Recently, the European Society of Cardiology's working group on myocardial and pericardial diseases recommended the use of NGS with very large panels of genes only when the family structure permits segregation analysis.<a class="elsevierStyleCrossRef" href="#bib0100"><span class="elsevierStyleSup">1</span></a></p><p id="par0010" class="elsevierStylePara elsevierViewall">Few studies to date have specifically assessed the genetic background of cohorts of HT recipients due to DCM,<a class="elsevierStyleCrossRefs" href="#bib0110"><span class="elsevierStyleSup">3,8,9</span></a> and even fewer have used NGS approaches.<a class="elsevierStyleCrossRef" href="#bib0145"><span class="elsevierStyleSup">10</span></a> Such studies are essential to provide data on the clinical utility of genetic tests in non-responsive DCM patients.</p><p id="par0015" class="elsevierStylePara elsevierViewall">In this work we aim to contribute to knowledge of genetic variants in adult patients undergoing HT due to end-stage DCM, reporting the results obtained in our single-center tertiary hospital series and using target NGS.</p></span><span id="sec0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0060">Methods</span><span id="sec0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0065">Population</span><p id="par0020" class="elsevierStylePara elsevierViewall">The study cohort comprised HT recipients with end-stage DCM (all with left ventricular ejection fraction [LVEF] <30%) from a single tertiary referral hospital.</p><p id="par0025" class="elsevierStylePara elsevierViewall">The study is part of a larger Portuguese project which aims to collect clinical and molecular data on adult patients with DCM.<a class="elsevierStyleCrossRef" href="#bib0150"><span class="elsevierStyleSup">11</span></a> DCM was classified as familial when idiopathic disease was present in more than one family member or when unexplained sudden cardiac death occurred under the age of 35 in at least one first-degree.</p><p id="par0030" class="elsevierStylePara elsevierViewall">General clinical assessment methods, such as electrocardiogram, echocardiogram and 24-hour Holter monitoring, have been previously reported.<a class="elsevierStyleCrossRef" href="#bib0150"><span class="elsevierStyleSup">11</span></a> In this patient subgroup, cardiac catheterization was performed in all cases.</p></span><span id="sec0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0070">Next-generation sequencing</span><p id="par0035" class="elsevierStylePara elsevierViewall">All patients provided a peripheral blood sample for molecular analysis. The samples were sent to a central laboratory, where DNA was extracted for molecular analysis and stored at -70<span class="elsevierStyleHsp" style=""></span>°C.</p><p id="par0040" class="elsevierStylePara elsevierViewall">Genetic variants in 15 genes – <span class="elsevierStyleItalic">LMNA</span>/C, <span class="elsevierStyleItalic">MYH7</span>, <span class="elsevierStyleItalic">MYBPC3</span>, <span class="elsevierStyleItalic">TNNT2</span>, <span class="elsevierStyleItalic">ACTA1</span>, <span class="elsevierStyleItalic">TPM1</span>, <span class="elsevierStyleItalic">CSRP3</span>, <span class="elsevierStyleItalic">TCAP</span>, <span class="elsevierStyleItalic">SGCD</span>, <span class="elsevierStyleItalic">PLN</span>, <span class="elsevierStyleItalic">MYL2</span>, <span class="elsevierStyleItalic">MYL3</span>, <span class="elsevierStyleItalic">TNNI3</span>, <span class="elsevierStyleItalic">TAZ</span> and <span class="elsevierStyleItalic">LDB3</span> – were screened in all samples, using polymerase chain reaction (PCR) with direct sequencing (NGS with at least 30-fold coverage combined with Sanger sequencing). Genes were preselected based on genetic variants previously identified in DCM patients.</p><p id="par0045" class="elsevierStylePara elsevierViewall">Primers were designed for all coding exons, covering exon-intron boundaries using the open-source software Primer3. No known variants were present in the primer sequences (dbSNP build 130). A multiplex PCR-based method was used to reduce the number of amplification reactions (primer sequences can be provided on request). Multiplex PCR reactions were performed following the QIAGEN Multiplex PCR Kit protocol (QIAGEN, Hilden, Germany).</p></span><span id="sec0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0075">Library and template preparation</span><p id="par0050" class="elsevierStylePara elsevierViewall">The quality of patient genomic DNA samples was evaluated by gel electrophoresis and quantification was performed using the Qubit dsDNA HS Assay Kit (Life Technologies). A total of 50 ng of genomic DNA was used in each multiplex PCR reaction. Libraries were prepared using the Ion Xpress™ Plus gDNA protocol and Amplicon Library Preparation (part number 4471989 Rev. C) (Life Technologies). Libraries were quantified using the Qubit dsDNA HS Assay. All libraries were diluted to the same concentration and pooled to ensure equal representation of the different samples. The diluted and combined libraries underwent amplification by emulsion PCR using the Ion PI™ Template OT2 200 Kit (Life Technologies) on an Ion OneTouch 2 Instrument (Life Technologies) according to the manufacturer's instructions. Ion Sphere particles were enriched using the Ion OneTouch 2 enrichment system (Life Technologies).</p></span><span id="sec0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0080">Semiconductor sequencing and data analysis</span><p id="par0055" class="elsevierStylePara elsevierViewall">Sequencing was carried out on an Ion PGM system using semiconductor technology. The Ion Sequencing Kit, version 2.0 (Life Technologies) was used to perform sequencing runs, following the manufacturer's recommendations. Data from the PGM runs were processed using the platform-specific Ion Torrent Suite pipeline software, version 4.2 (Life Technologies) to generate sequence reads, trim adapter sequences, filter and remove poor signal reads, and split the reads according to the barcode. Reads were assembled with SeqMan NGen, version 4.1 (DNASTAR, Madison, WI) using the FASTQ files containing sequence reads, and the template references were adjusted for the covered amplicons. SeqMan Pro version 10 (DNASTAR) was used for post-assembly analysis of the overall amplicon coverage, individual base depth of coverage, and variant identification.</p><p id="par0060" class="elsevierStylePara elsevierViewall">Pathogenicity was assessed by comparing the results with databases in which genetic variants had been reported, as well as using predictive models and segregation analysis, in accordance with the guidelines of the American College of Medical Genetics and Genomics and the Association for Molecular Pathology.<a class="elsevierStyleCrossRef" href="#bib0155"><span class="elsevierStyleSup">12</span></a></p><p id="par0065" class="elsevierStylePara elsevierViewall">Subsequently, the patients’ relatives were screened for the identified genetic variants. Clinical assessment, electrocardiogram and echocardiogram were performed in all available family members.</p></span><span id="sec0035" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0085">Ethics</span><p id="par0070" class="elsevierStylePara elsevierViewall">Informed consent was obtained from all patients and relatives. The study protocol conforms to the ethical guidelines of the 1975 Declaration of Helsinki as confirmed by prior approval by the institution's human research committee.</p></span></span><span id="sec0040" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0090">Results</span><span id="sec0045" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0095">Clinical characteristics</span><p id="par0075" class="elsevierStylePara elsevierViewall">Thirteen unrelated patients were included, nine (69%) male, with a mean age at DCM diagnosis of 33±13 years, eight (62%) with familial DCM.</p><p id="par0080" class="elsevierStylePara elsevierViewall">Native heart LVEF was 18.2±5.8% and LV end-diastolic diameter was 67.5±10.2 mm. Five patients (38%) presented with left bundle branch block and three were in non-sinus rhythm, two with atrial standstill. Cardiac magnetic resonance imaging was performed in four patients (31%) and endomyocardial biopsy in two, which revealed non-specific alterations. Elevation of serum creatine kinase in at least one laboratory assessment was detected in three (23%) cases, in two of which neuromuscular assessment confirmed the presence of skeletal myopathy (<a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a>). Seven patients (54%) had received an implantable cardioverter-defibrillator (ICD) and three (23%) an LV resynchronization device before HT.</p><elsevierMultimedia ident="tbl0005"></elsevierMultimedia><p id="par0085" class="elsevierStylePara elsevierViewall">We were able to assess 35 relatives of eight of the probands, of whom six (17%) presented DCM and two (6%) previously documented LV systolic dysfunction, from which they recovered (reverse remodeling).</p></span><span id="sec0050" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0100">Genetic analysis</span><p id="par0090" class="elsevierStylePara elsevierViewall">Nine genetic variants were identified in six (46%) patients: five in <span class="elsevierStyleItalic">LMNA</span>, two in <span class="elsevierStyleItalic">LBD3</span>, one in <span class="elsevierStyleItalic">TNNT2</span> and one in <span class="elsevierStyleItalic">TCAP</span>. These variants were new in most patients. Two patients (15%) were double and triple heterozygotes in the <span class="elsevierStyleItalic">LBD3</span> and <span class="elsevierStyleItalic">LMNA</span> genes, respectively. The majority were classified as of uncertain significance. Only one variant, in <span class="elsevierStyleItalic">LMNA</span>, was classified as likely pathogenic. This variant, in exon 6 of <span class="elsevierStyleItalic">LMNA</span> (c.1003C>T), leads to an amino acid substitution at position 335 of the protein (p.Arg335Trp) and has been reported in other DCM patients.<a class="elsevierStyleCrossRef" href="#bib0160"><span class="elsevierStyleSup">13</span></a><a class="elsevierStyleCrossRefs" href="#fig0005">Figures 1-3</a>, respectively, show the baseline ECG, pathologic features of the explanted heart, and the genogram of patient 3, in whom this variant was identified. Genetic family screening enabled familial non-segregation of the <span class="elsevierStyleItalic">TCAP</span> variant to be established. <a class="elsevierStyleCrossRef" href="#tbl0010">Table 2</a> depicts the final classification of the genetic variants detected in the present study.</p><elsevierMultimedia ident="fig0005"></elsevierMultimedia><elsevierMultimedia ident="fig0010"></elsevierMultimedia><elsevierMultimedia ident="fig0015"></elsevierMultimedia><elsevierMultimedia ident="tbl0010"></elsevierMultimedia></span></span><span id="sec0055" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0105">Discussion</span><p id="par0095" class="elsevierStylePara elsevierViewall">DCM is a major indication for HT, and a lower LVEF indicates a higher risk for this therapeutic option.<a class="elsevierStyleCrossRef" href="#bib0165"><span class="elsevierStyleSup">14</span></a> However, HT is not necessarily a definitive treatment, due to complications arising from graft failure and chronic immunosuppression. The median life expectancy of transplant patients is still around 15 years.<a class="elsevierStyleCrossRef" href="#bib0170"><span class="elsevierStyleSup">15</span></a> At the same time, insufficient donor hearts are available for all candidates and many patients die while on the waiting list. Given this situation, it is essential to further explore the mechanisms behind failure to respond to heart failure therapies in patients who progress to end-stage disease.</p><p id="par0100" class="elsevierStylePara elsevierViewall">Genetics appears to explain a significant proportion of DCM cases. However, in most HT recipients, due to the scarcity of specific etiologic markers and the fact that genetic alterations are not systematically analyzed in these patients, DCM is frequently categorized as idiopathic. Systemic manifestations can occasionally alert the clinician to the possibility of a genetic disease, such as the presence of skeletal myopathy or atrioventricular block in carriers of <span class="elsevierStyleItalic">LMNA</span> variants.</p><p id="par0105" class="elsevierStylePara elsevierViewall">The recently developed NGS technologies are more effective in detecting genetic variants in HT candidates, but the most efficient way to use NGS in clinical practice remains to be established, in particular how to select the panel of genes to be included in a target gene approach.</p><p id="par0110" class="elsevierStylePara elsevierViewall">The few reports of genetic yields obtained specifically in HT cohorts of DCM patients use different approaches. Initial studies with Sanger DNA sequencing revealed a relatively high frequency of <span class="elsevierStyleItalic">LMNA</span> variants,<a class="elsevierStyleCrossRef" href="#bib0110"><span class="elsevierStyleSup">3</span></a> while more recently, Cuenca et al., after screening a significant number of genes by NGS, reported pathogenic variants in 40% of patients, the most frequent in emerin and titin genes.<a class="elsevierStyleCrossRef" href="#bib0145"><span class="elsevierStyleSup">10</span></a></p><p id="par0115" class="elsevierStylePara elsevierViewall">In our study, genetic variants were a common finding (46%), but only one variant was categorized as likely pathogenic, due to the small and non-informative families. We also found a high percentage of <span class="elsevierStyleItalic">LMNA</span> variants (23%), followed by variants in <span class="elsevierStyleItalic">LBD3</span> and <span class="elsevierStyleItalic">TNNT2</span>. The patient with the pathogenic <span class="elsevierStyleItalic">LMNA</span> variant presented elevated serum creatine kinase, but this observation was only detected once, and has not been associated with overt clinical myopathy. This missense variant, located in the coil 2 domain of the lamin A protein, has also been reported in another DCM patient with no evidence of muscular dystrophy, who required an ICD due to ventricular tachycardia and underwent HT at the age of 39.<a class="elsevierStyleCrossRef" href="#bib0160"><span class="elsevierStyleSup">13</span></a></p><p id="par0120" class="elsevierStylePara elsevierViewall">Two other patients presented stable rigid spine syndrome and limb-girdle myopathy, respectively, but in neither case did skeletal involvement limit HT. These cases highlight the well-known importance of neuromuscular assessment in DCM patients.<a class="elsevierStyleCrossRef" href="#bib0175"><span class="elsevierStyleSup">16</span></a></p><p id="par0125" class="elsevierStylePara elsevierViewall">Concerning the presence of two or more genetic variants in the same individual, it is not known if or how this may modify the phenotype. Double heterozygosity for <span class="elsevierStyleItalic">LMNA</span> and titin variants has been shown to be associated with a more severe clinical course, in terms of age of terminal heart failure and HT in a DCM family.<a class="elsevierStyleCrossRef" href="#bib0180"><span class="elsevierStyleSup">17</span></a> Compound and digenic heterozygosity also appear to be common among hypertrophic cardiomyopathy (HCM) patients who develop end-stage heart failure.<a class="elsevierStyleCrossRef" href="#bib0185"><span class="elsevierStyleSup">18</span></a> Of note, it is possible that end-stage DCM may represent end-stage HCM, but this is less likely in our patients, given the follow-up before HT, and since histopathology of the explanted hearts did not reveal myofibril disarray in any of the cases. On the other hand, the lack of segregation of genetic variants does not mean they have no biological effects, particularly when detected in family members with more severe phenotypes.</p><p id="par0130" class="elsevierStylePara elsevierViewall">Major limitations of our study are the small number of patients and the limited number of studied genes, especially the fact that we did not include titin, in which genetic variants appear to have an important role in the pathogenesis of DCM.<a class="elsevierStyleCrossRef" href="#bib0190"><span class="elsevierStyleSup">19</span></a></p></span><span id="sec0060" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0110">Conclusions</span><p id="par0135" class="elsevierStylePara elsevierViewall">The application of NGS may help to expand our knowledge of the genetic mechanisms of cardiomyopathies that do not respond to medical therapy, including in DCM patients undergoing HT. It is important to clarify the pathogenicity of identified variants, and until more data are available, most variants will be classified as of uncertain significance, as in our patients. The impact of compound or digenic heterozygosity should also be investigated, in view of the growing number of clinical and genetic reports of patients with such mutations.</p><p id="par0140" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleItalic">LMNA</span> is one of the most frequently mutated genes in HT patients and should be included in all target gene assessments of end-stage DCM patients.</p></span><span id="sec0065" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0115">Conflicts of interest</span><p id="par0145" class="elsevierStylePara elsevierViewall">The authors have no conflicts of interest to declare.</p></span></span>" "textoCompletoSecciones" => array:1 [ "secciones" => array:12 [ 0 => array:3 [ "identificador" => "xres1269319" "titulo" => "Abstract" "secciones" => array:3 [ 0 => array:2 [ "identificador" => "abst0005" "titulo" => "Introduction and Objectives" ] 1 => array:2 [ "identificador" => "abst0010" "titulo" => "Methods and Results" ] 2 => array:2 [ "identificador" => "abst0015" "titulo" => "Conclusion" ] ] ] 1 => array:2 [ "identificador" => "xpalclavsec1174834" "titulo" => "Keywords" ] 2 => array:3 [ "identificador" => "xres1269320" "titulo" => "Resumo" "secciones" => array:3 [ 0 => array:2 [ "identificador" => "abst0020" "titulo" => "Introdução e objetivos" ] 1 => array:2 [ "identificador" => "abst0025" "titulo" => "Métodos e resultados" ] 2 => array:2 [ "identificador" => "abst0030" "titulo" => "Conclusões" ] ] ] 3 => array:2 [ "identificador" => "xpalclavsec1174835" "titulo" => "Palavras-chave" ] 4 => array:2 [ "identificador" => "sec0005" "titulo" => "Introduction" ] 5 => array:3 [ "identificador" => "sec0010" "titulo" => "Methods" "secciones" => array:5 [ 0 => array:2 [ "identificador" => "sec0015" "titulo" => "Population" ] 1 => array:2 [ "identificador" => "sec0020" "titulo" => "Next-generation sequencing" ] 2 => array:2 [ "identificador" => "sec0025" "titulo" => "Library and template preparation" ] 3 => array:2 [ "identificador" => "sec0030" "titulo" => "Semiconductor sequencing and data analysis" ] 4 => array:2 [ "identificador" => "sec0035" "titulo" => "Ethics" ] ] ] 6 => array:3 [ "identificador" => "sec0040" "titulo" => "Results" "secciones" => array:2 [ 0 => array:2 [ "identificador" => "sec0045" "titulo" => "Clinical characteristics" ] 1 => array:2 [ "identificador" => "sec0050" "titulo" => "Genetic analysis" ] ] ] 7 => array:2 [ "identificador" => "sec0055" "titulo" => "Discussion" ] 8 => array:2 [ "identificador" => "sec0060" "titulo" => "Conclusions" ] 9 => array:2 [ "identificador" => "sec0065" "titulo" => "Conflicts of interest" ] 10 => array:2 [ "identificador" => "xack435589" "titulo" => "Acknowledgments/Funding Sources" ] 11 => array:1 [ "titulo" => "References" ] ] ] "pdfFichero" => "main.pdf" "tienePdf" => true "fechaRecibido" => "2017-06-02" "fechaAceptado" => "2019-02-03" "PalabrasClave" => array:2 [ "en" => array:1 [ 0 => array:4 [ "clase" => "keyword" "titulo" => "Keywords" "identificador" => "xpalclavsec1174834" "palabras" => array:4 [ 0 => "Dilated cardiomyopathy" 1 => "Heart transplantation" 2 => "Next-generation sequencing" 3 => "Genetic variants" ] ] ] "pt" => array:1 [ 0 => array:4 [ "clase" => "keyword" "titulo" => "Palavras-chave" "identificador" => "xpalclavsec1174835" "palabras" => array:4 [ 0 => "Miocardiopatia dilatada" 1 => "Transplante cardíaco" 2 => "Sequenciação de nova geração" 3 => "Variantes genéticas" ] ] ] ] "tieneResumen" => true "resumen" => array:2 [ "en" => array:3 [ "titulo" => "Abstract" "resumen" => "<span id="abst0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0010">Introduction and Objectives</span><p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">Dilated cardiomyopathy (DCM) is a myocardial disease that can progress to a terminal stage, requiring heart transplantation. In this work we aim to contribute to knowledge of genetic variants in adult patients undergoing heart transplantation due to end-stage DCM, reporting the results obtained in our single-center tertiary hospital series using target next-generation sequencing (NGS).</p></span> <span id="abst0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0015">Methods and Results</span><p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">Genetic variants were screened in 15 genes, preselected based on variants previously identified in DCM patients. Thirteen unrelated patients were included, nine (69%) male, mean age at diagnosis 33±13 years, eight (62%) with familial DCM. Nine genetic variants were identified in six (46%) patients: five in <span class="elsevierStyleItalic">LMNA</span>, two in <span class="elsevierStyleItalic">LBD3</span>, one in <span class="elsevierStyleItalic">TNNT2</span> and one in <span class="elsevierStyleItalic">TCAP</span>. These variants were new in most patients. The majority were classified as of uncertain significance. Two patients were double and triple heterozygotes in the <span class="elsevierStyleItalic">LBD3</span> and <span class="elsevierStyleItalic">LMNA</span> genes, respectively.</p></span> <span id="abst0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0020">Conclusion</span><p id="spar0015" class="elsevierStyleSimplePara elsevierViewall">Our results highlight the potential of NGS in the genetic characterization of DCM patients. <span class="elsevierStyleItalic">LMNA</span> is one of the most frequently mutated genes and should be included in all target gene assessments of end-stage DCM patients until more data are available.</p></span>" "secciones" => array:3 [ 0 => array:2 [ "identificador" => "abst0005" "titulo" => "Introduction and Objectives" ] 1 => array:2 [ "identificador" => "abst0010" "titulo" => "Methods and Results" ] 2 => array:2 [ "identificador" => "abst0015" "titulo" => "Conclusion" ] ] ] "pt" => array:3 [ "titulo" => "Resumo" "resumen" => "<span id="abst0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0030">Introdução e objetivos</span><p id="spar0020" class="elsevierStyleSimplePara elsevierViewall">A miocardiopatia dilatada é uma doença miocárdica que pode evoluir para um estádio terminal, requerendo transplante cardíaco. Neste trabalho, pretendemos contribuir para o conhecimento das variantes genéticas presentes em pacientes adultos submetidos a transplante cardíaco, descrevendo os resultados obtidos utilizando técnicas de sequenciação de ADN de nova geração.</p></span> <span id="abst0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0035">Métodos e resultados</span><p id="spar0025" class="elsevierStyleSimplePara elsevierViewall">Variantes genéticas foram pesquisadas em 15 genes pré-selecionados com base em variantes previamente identificadas em pacientes com miocardiopatia dilatada. Foram incluídos 13 pacientes não relacionados, nove (69%) do sexo masculino, com idade média na altura do diagnóstico de 33±13 anos, oito (62%) com doença familiar. Foram identificadas nove variantes genéticas em seis (46%) pacientes: LMNA-5, LBD3-2, TNNT2-1 e TCAP- 1. A maioria das variantes genéticas foi classificada como de significado incerto. Dois pacientes eram heterozigotos duplos e triplos nos genes LBD3 e LMNA, respetivamente.</p></span> <span id="abst0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0040">Conclusões</span><p id="spar0030" class="elsevierStyleSimplePara elsevierViewall">Os nossos resultados reforçam o potencial das novas tecnologias de sequenciação na caracterização genética de doentes com miocardiopatia dilatada. Até que mais dados estejam disponíveis, o gene LMNA é um dos mais frequentemente envolvidos e deverá ser incluído na avaliação de pacientes com miocardiopatia dilatada em fase terminal.</p></span>" "secciones" => array:3 [ 0 => array:2 [ "identificador" => "abst0020" "titulo" => "Introdução e objetivos" ] 1 => array:2 [ "identificador" => "abst0025" "titulo" => "Métodos e resultados" ] 2 => array:2 [ "identificador" => "abst0030" "titulo" => "Conclusões" ] ] ] ] "multimedia" => array:5 [ 0 => array:7 [ "identificador" => "fig0005" "etiqueta" => "Figure 1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr1.jpeg" "Alto" => 369 "Ancho" => 1405 "Tamanyo" => 105451 ] ] "descripcion" => array:1 [ "en" => "<p id="spar0035" class="elsevierStyleSimplePara elsevierViewall">Patient 3: baseline 12-lead electrocardiogram and 24-hour Holter monitoring showing left ventricular hypertrophy (A) and runs of nonsustained ventricular tachycardia (B).</p>" ] ] 1 => array:7 [ "identificador" => "fig0010" "etiqueta" => "Figure 2" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr2.jpeg" "Alto" => 925 "Ancho" => 1255 "Tamanyo" => 180540 ] ] "descripcion" => array:1 [ "en" => "<p id="spar0040" class="elsevierStyleSimplePara elsevierViewall">Patient 3: pathologic features of the explanted heart. (A) Macroscopy of heart section revealing features of dilated cardiomyopathy; (B) microscopic features (H&E stain) revealing interstitial fibrosis, hypertrophic cardiomyocytes and scattered adipocytes (left: low power; right: high power).</p>" ] ] 2 => array:7 [ "identificador" => "fig0015" "etiqueta" => "Figure 3" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr3.jpeg" "Alto" => 2097 "Ancho" => 1508 "Tamanyo" => 96679 ] ] "descripcion" => array:1 [ "en" => "<p id="spar0045" class="elsevierStyleSimplePara elsevierViewall">Patient 3 genogram. Square: male; circle: female; dark symbol: dilated cardiomyopathy; +/-: presence/absence of <span class="elsevierStyleItalic">LMNA</span> c.1003C>T variant; numbers inside symbols: current age (years); ↑ sCK: elevated serum creatine kinase; CA: cardiac assessment; HT: heart transplantation; ?: unavailable for clinical/genetic assessment.</p>" ] ] 3 => array:8 [ "identificador" => "tbl0005" "etiqueta" => "Table 1" "tipo" => "MULTIMEDIATABLA" "mostrarFloat" => true "mostrarDisplay" => false "detalles" => array:1 [ 0 => array:3 [ "identificador" => "at1" "detalle" => "Table " "rol" => "short" ] ] "tabla" => array:2 [ "leyenda" => "<p id="spar0055" class="elsevierStyleSimplePara elsevierViewall">CK: creatine kinase; CMRI: cardiac magnetic resonance imaging; DCM: dilated cardiomyopathy; ECG: electrocardiogram; HT: heart transplantation; LAH: left anterior hemiblock; LBBB: left bundle branch block; LE: late enhancement; LVED: left ventricular end-diastolic diameter; LVEF: left ventricular ejection fraction; LVH: left ventricular hypertrophy; MI: myocardial infarction; RBBB: right bundle branch block; SR: sinus rhythm.</p>" "tablatextoimagen" => array:1 [ 0 => array:2 [ "tabla" => array:1 [ 0 => """ <table border="0" frame="\n \t\t\t\t\tvoid\n \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><th class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" scope="col" style="border-bottom: 2px solid black">Patient \t\t\t\t\t\t\n \t\t\t\t\t\t</th><th class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" scope="col" style="border-bottom: 2px solid black">Gender/age, years \t\t\t\t\t\t\n \t\t\t\t\t\t</th><th class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" scope="col" style="border-bottom: 2px solid black">ECG before HT \t\t\t\t\t\t\n \t\t\t\t\t\t</th><th class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" scope="col" style="border-bottom: 2px solid black">Native heart LVEF, %/LVED, mm \t\t\t\t\t\t\n \t\t\t\t\t\t</th><th class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" scope="col" style="border-bottom: 2px solid black">Comments \t\t\t\t\t\t\n \t\t\t\t\t\t</th><th class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" scope="col" style="border-bottom: 2px solid black">Genetic variant identified \t\t\t\t\t\t\n \t\t\t\t\t\t</th></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">1 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">F/47 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">SR/- \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">15/65 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">Postpartum DCM diagnosis \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">No \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">2 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">M/55 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">SR/LBBB \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">8/87 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="" valign="\n \t\t\t\t\ttop\n \t\t\t\t"> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">Yes \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">3 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">M/44 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">SR/LVH \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">29/55 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">CK elevation, no evidence of skeletal myopathy; transmural apical and lateral LE on CMRI; no evidence of MI on explanted heart tissue \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">Yes \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">4 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">M/60 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">SR/LBBB \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">15/78 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">Intramyocardial mid-septal LE in on CMRI \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">Yes \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">5 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">M/56 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">Atrial flutter/LBBB \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">16/63 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="" valign="\n \t\t\t\t\ttop\n \t\t\t\t"> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">No \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">6 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">M/53 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">Atrial standstill/RBBB+LAH \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">10/59 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">CK elevation with statins, limb-girdle myopathy, skeletal biopsy with lobulated fibers \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">No \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">7 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">M/36 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">SR/LVH \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">23/70 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="" valign="\n \t\t\t\t\ttop\n \t\t\t\t"> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">Yes \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">8 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">F/32 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">SR/- \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">16/59 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">No LE on CMRI \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">No \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">9 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">M/61 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">SR/RBBB+LAH \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">23/67 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="" valign="\n \t\t\t\t\ttop\n \t\t\t\t"> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">No \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">10 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">M/60 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">Atrial standstill/ventricular pacing \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">24/58 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">CK elevation, rigid spine syndrome \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">Yes \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">11 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">F/26 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">SR/LVH \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">17/83 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">No LE on CMRI \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">No \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">12 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">M/47 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">SR/LBBB \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">21/60 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="" valign="\n \t\t\t\t\ttop\n \t\t\t\t"> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">Yes \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">13 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">F/53 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">SR/LBBB \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">20/74 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="" valign="\n \t\t\t\t\ttop\n \t\t\t\t"> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">No \t\t\t\t\t\t\n \t\t\t\t</td></tr></tbody></table> """ ] "imagenFichero" => array:1 [ 0 => "xTab2171161.png" ] ] ] ] "descripcion" => array:1 [ "en" => "<p id="spar0050" class="elsevierStyleSimplePara elsevierViewall">Clinical characteristics of the study population.</p>" ] ] 4 => array:8 [ "identificador" => "tbl0010" "etiqueta" => "Table 2" "tipo" => "MULTIMEDIATABLA" "mostrarFloat" => true "mostrarDisplay" => false "detalles" => array:1 [ 0 => array:3 [ "identificador" => "at2" "detalle" => "Table " "rol" => "short" ] ] "tabla" => array:2 [ "leyenda" => "<p id="spar0065" class="elsevierStyleSimplePara elsevierViewall">ACMG: American College of Medical Genetics and Genomics; NCBI: National Center for Biotechnology Information; VUS: variant of uncertain significance.</p>" "tablatextoimagen" => array:1 [ 0 => array:2 [ "tabla" => array:1 [ 0 => """ <table border="0" frame="\n \t\t\t\t\tvoid\n \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><th class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" scope="col" style="border-bottom: 2px solid black">Patient \t\t\t\t\t\t\n \t\t\t\t\t\t</th><th class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" scope="col" style="border-bottom: 2px solid black">Gene \t\t\t\t\t\t\n \t\t\t\t\t\t</th><th class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" scope="col" style="border-bottom: 2px solid black">NM (NCBI) \t\t\t\t\t\t\n \t\t\t\t\t\t</th><th class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" scope="col" style="border-bottom: 2px solid black">Variant \t\t\t\t\t\t\n \t\t\t\t\t\t</th><th class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" scope="col" style="border-bottom: 2px solid black">Final classification (ACMG) \t\t\t\t\t\t\n \t\t\t\t\t\t</th></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td-with-role" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">310101012 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="middle"><span class="elsevierStyleItalic">LMNA</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t"><span class="elsevierStyleInterRef" id="intr0005" href="ncbi-n:NM_170707.3">NM_170707.3</span><span class="elsevierStyleInterRef" id="intr0010" href="ncbi-n:NM_170707.3">NM_170707.3</span><span class="elsevierStyleInterRef" id="intr0015" href="ncbi-n:NM_170707.3">NM_170707.3</span><span class="elsevierStyleInterRef" id="intr0020" href="ncbi-n:NM_170707.3">NM_170707.3</span><span class="elsevierStyleInterRef" id="intr0025" href="ncbi-n:NM_170707.3">NM_170707.3</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">c.1003C>T; p.Arg335Trpc.1318G>A; p.Val440Metc.1604G>C; p.Gly535Alac.1982G>C; p.Cys661Serc.460G>A; p.Glu154Lys \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">Likely pathogenicVUSVUSVUSVUS \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">44 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="middle"><span class="elsevierStyleItalic">LBD3</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t"><span class="elsevierStyleInterRef" id="intr0030" href="ncbi-n:NM_007078.2">NM_007078.2</span><span class="elsevierStyleInterRef" id="intr0035" href="ncbi-n:NM_007078.2">NM_007078.2</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">c.466G>A; p.Ala156Thrc.1189G>A; p.Val397Ile \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">VUSVUS \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">7 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t"><span class="elsevierStyleItalic">TNNT2</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t"><span class="elsevierStyleInterRef" id="intr0040" href="ncbi-n:NM_001001430.2">NM_001001430.2</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">c.325C>T; p.His109Tyr \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">VUS \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">2 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t"><span class="elsevierStyleItalic">TCAP</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t"><span class="elsevierStyleInterRef" id="intr0045" href="ncbi-n:NM_003673.3">NM_003673.3</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">c.313G>C; p.Glu105Gln \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">VUS \t\t\t\t\t\t\n \t\t\t\t</td></tr></tbody></table> """ ] "imagenFichero" => array:1 [ 0 => "xTab2171162.png" ] ] ] ] "descripcion" => array:1 [ "en" => "<p id="spar0060" class="elsevierStyleSimplePara elsevierViewall">Classification of genetic variants detected (in accordance with the guidelines of the American College of Medical Genetics and Genomics and the Association for Molecular Pathology<a class="elsevierStyleCrossRef" href="#bib0155"><span class="elsevierStyleSup">12</span></a>).</p>" ] ] ] "bibliografia" => array:2 [ "titulo" => "References" "seccion" => array:1 [ 0 => array:2 [ "identificador" => "bibs0015" "bibliografiaReferencia" => array:19 [ 0 => array:3 [ "identificador" => "bib0100" "etiqueta" => "1" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Proposal for a revised definition of dilated cardiomyopathy, hypokinetic non-dilated cardiomyopathy, and its implications for clinical practice: a position statement of the ESC working group on myocardial and pericardial diseases" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:3 [ 0 => "Y.M. Pinto" 1 => "P.M. Elliott" 2 => "E. Arbustini" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1093/eurheartj/ehv727" "Revista" => array:6 [ "tituloSerie" => "Eur Heart J" "fecha" => "2016" "volumen" => "37" "paginaInicial" => "1850" "paginaFinal" => "1858" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/26792875" "web" => "Medline" ] ] ] ] ] ] ] ] 1 => array:3 [ "identificador" => "bib0105" "etiqueta" => "2" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Classification of the cardiomyopathies" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:3 [ 0 => "P. Elliott" 1 => "B. Andersson" 2 => "E. Arbustini" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Kardiol Pol" "fecha" => "2008" "volumen" => "66" "paginaInicial" => "533" "paginaFinal" => "540" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18630392" "web" => "Medline" ] ] ] ] ] ] ] ] 2 => array:3 [ "identificador" => "bib0110" "etiqueta" => "3" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Novel mutations in the lamin A/C gene in heart transplant recipients with end stage dilated cardiomyopathy" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:3 [ 0 => "S. Karkkainen" 1 => "E. Reissell" 2 => "T. Helio" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1136/hrt.2004.056721" "Revista" => array:6 [ "tituloSerie" => "Heart" "fecha" => "2006" "volumen" => "92" "paginaInicial" => "524" "paginaFinal" => "526" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16537768" "web" => "Medline" ] ] ] ] ] ] ] ] 3 => array:3 [ "identificador" => "bib0115" "etiqueta" => "4" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Genetics and heart failure: a concise guide for the clinician" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:3 [ 0 => "C. Skrzynia" 1 => "J.S. Berg" 2 => "M.S. Willis" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.2174/1573403x09666131117170446" "Revista" => array:6 [ "tituloSerie" => "Curr Cardiol Rev" "fecha" => "2015" "volumen" => "11" "paginaInicial" => "10" "paginaFinal" => "17" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/24251456" "web" => "Medline" ] ] ] ] ] ] ] ] 4 => array:3 [ "identificador" => "bib0120" "etiqueta" => "5" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Truncations of titin causing dilated cardiomyopathy" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:3 [ 0 => "D.S. Herman" 1 => "L. Lam" 2 => "M.R. Taylor" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1056/NEJMoa1110186" "Revista" => array:6 [ "tituloSerie" => "N Engl J Med" "fecha" => "2012" "volumen" => "366" "paginaInicial" => "619" "paginaFinal" => "628" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/22335739" "web" => "Medline" ] ] ] ] ] ] ] ] 5 => array:3 [ "identificador" => "bib0125" "etiqueta" => "6" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Atlas of the clinical genetics of human dilated cardiomyopathy" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:3 [ 0 => "J. Haas" 1 => "K.S. Frese" 2 => "B. Peil" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:3 [ "tituloSerie" => "Eur Heart J" "fecha" => "2015" "volumen" => "36" ] ] ] ] ] ] 6 => array:3 [ "identificador" => "bib0130" "etiqueta" => "7" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Massive parallel sequencing questions the pathogenic role of missense variants in dilated cardiomyopathy" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:3 [ 0 => "M.G. Dalin" 1 => "P.G. Engstrom" 2 => "E.G. Ivarsson" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1016/j.ijcard.2016.11.066" "Revista" => array:6 [ "tituloSerie" => "Int J Cardiol" "fecha" => "2016" "volumen" => "228" "paginaInicial" => "742" "paginaFinal" => "748" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/27886618" "web" => "Medline" ] ] ] ] ] ] ] ] 7 => array:3 [ "identificador" => "bib0135" "etiqueta" => "8" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Familial dilated cardiomyopathy in patients transplanted for idiopathic dilated cardiomyopathy" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:3 [ 0 => "L. Monserrat" 1 => "M. Hermida" 2 => "B. Bouzas" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1016/s0300-8932(02)76691-8" "Revista" => array:6 [ "tituloSerie" => "Rev Esp Cardiol" "fecha" => "2002" "volumen" => "55" "paginaInicial" => "725" "paginaFinal" => "732" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/12113700" "web" => "Medline" ] ] ] ] ] ] ] ] 8 => array:3 [ "identificador" => "bib0140" "etiqueta" => "9" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Desmosomal protein gene mutations in patients with idiopathic dilated cardiomyopathy undergoing cardiac transplantation: a clinicopathological study" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:3 [ 0 => "P. Garcia-Pavia" 1 => "P. Syrris" 2 => "C. Salas" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1136/hrt.2011.227967" "Revista" => array:6 [ "tituloSerie" => "Heart" "fecha" => "2011" "volumen" => "97" "paginaInicial" => "1744" "paginaFinal" => "1752" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/21859740" "web" => "Medline" ] ] ] ] ] ] ] ] 9 => array:3 [ "identificador" => "bib0145" "etiqueta" => "10" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Genetic basis of familial dilated cardiomyopathy patients undergoing heart transplantation" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:3 [ 0 => "S. Cuenca" 1 => "M.J. Ruiz-Cano" 2 => "J.R. Gimeno-Blanes" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1016/j.healun.2015.12.014" "Revista" => array:6 [ "tituloSerie" => "J Heart Lung Transplant" "fecha" => "2016" "volumen" => "35" "paginaInicial" => "625" "paginaFinal" => "635" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/26899768" "web" => "Medline" ] ] ] ] ] ] ] ] 10 => array:3 [ "identificador" => "bib0150" "etiqueta" => "11" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Portuguese study of familial dilated cardiomyopathy: the FATIMA study" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:3 [ 0 => "E. Martins" 1 => "J. Silva-Cardoso" 2 => "M. Bicho" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Rev Port Cardiol" "fecha" => "2008" "volumen" => "27" "paginaInicial" => "1029" "paginaFinal" => "1042" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/19044174" "web" => "Medline" ] ] ] ] ] ] ] ] 11 => array:3 [ "identificador" => "bib0155" "etiqueta" => "12" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Standards and guidelines for the interpretation of sequence variants: a joint consensus recommendation of the American College of Medical Genetics and Genomics and the Association for Molecular Pathology" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:3 [ 0 => "S. Richards" 1 => "N. Aziz" 2 => "S. Bale" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1038/gim.2015.30" "Revista" => array:6 [ "tituloSerie" => "Genet Med" "fecha" => "2015" "volumen" => "17" "paginaInicial" => "405" "paginaFinal" => "424" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/25741868" "web" => "Medline" ] ] ] ] ] ] ] ] 12 => array:3 [ "identificador" => "bib0160" "etiqueta" => "13" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Identification of novel mutations in LMNA associated with familial forms of dilated cardiomyopathy" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:3 [ 0 => "B. Stallmeyer" 1 => "M. Koopmann" 2 => "E. Schulze-Bahr" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:5 [ "tituloSerie" => "Genet Test Mol Biomark" "fecha" => "2012" "volumen" => "16" "paginaInicial" => "543" "paginaFinal" => "549" ] ] ] ] ] ] 13 => array:3 [ "identificador" => "bib0165" "etiqueta" => "14" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:1 [ "autores" => array:1 [ 0 => array:3 [ "colaboracion" => "Competence Network Heart Failure Germany. Long-term outcome and predictors of outcome in patients with non-ischemic dilated cardiomyopathy" "etal" => true "autores" => array:3 [ 0 => "K. Karatolios" 1 => "V. Holzendorf" 2 => "A. Richter" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1016/j.ijcard.2016.06.167" "Revista" => array:6 [ "tituloSerie" => "Int J Cardiol" "fecha" => "2016" "volumen" => "220" "paginaInicial" => "608" "paginaFinal" => "612" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/27390998" "web" => "Medline" ] ] ] ] ] ] ] ] 14 => array:3 [ "identificador" => "bib0170" "etiqueta" => "15" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Matching the market for heart transplantation" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "E.M. Hsich" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1161/CIRCHEARTFAILURE.115.002679" "Revista" => array:5 [ "tituloSerie" => "Circ Heart Fail" "fecha" => "2016" "volumen" => "9" "paginaInicial" => "e002679" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/27072859" "web" => "Medline" ] ] ] ] ] ] ] ] 15 => array:3 [ "identificador" => "bib0175" "etiqueta" => "16" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Prevalent cardiac phenotype resulting in heart transplantation in a novel LMNA gene duplication" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:3 [ 0 => "L. Volpi" 1 => "G. Ricci" 2 => "C. Passino" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1016/j.nmd.2010.03.016" "Revista" => array:6 [ "tituloSerie" => "Neuromuscul Disord" "fecha" => "2010" "volumen" => "20" "paginaInicial" => "512" "paginaFinal" => "516" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/20580235" "web" => "Medline" ] ] ] ] ] ] ] ] 16 => array:3 [ "identificador" => "bib0180" "etiqueta" => "17" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Doubly heterozygous LMNA and TTN mutations revealed by exome sequencing in a severe form of dilated cardiomyopathy" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:3 [ 0 => "R. Roncarati" 1 => "C. Viviani Anselmi" 2 => "P. Krawitz" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1038/ejhg.2013.16" "Revista" => array:6 [ "tituloSerie" => "Eur J Hum Genet" "fecha" => "2013" "volumen" => "21" "paginaInicial" => "1105" "paginaFinal" => "1111" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/23463027" "web" => "Medline" ] ] ] ] ] ] ] ] 17 => array:3 [ "identificador" => "bib0185" "etiqueta" => "18" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Genetic basis of end-stage hypertrophic cardiomyopathy" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:3 [ 0 => "P. Garcia-Pavia" 1 => "M.E. Vazquez" 2 => "J. Segovia" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1093/eurjhf/hfr110" "Revista" => array:6 [ "tituloSerie" => "Eur J Heart Fail" "fecha" => "2011" "volumen" => "13" "paginaInicial" => "1193" "paginaFinal" => "1201" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/21896538" "web" => "Medline" ] ] ] ] ] ] ] ] 18 => array:3 [ "identificador" => "bib0190" "etiqueta" => "19" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Prevalence of titin truncating variants in general population" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:3 [ 0 => "O. Akinrinade" 1 => "J.W. Koskenvuo" 2 => "T.P. Alastalo" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1371/journal.pone.0145284" "Revista" => array:5 [ "tituloSerie" => "PLOS ONE" "fecha" => "2015" "volumen" => "10" "paginaInicial" => "e0145284" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/26701604" "web" => "Medline" ] ] ] ] ] ] ] ] ] ] ] ] "agradecimientos" => array:1 [ 0 => array:4 [ "identificador" => "xack435589" "titulo" => "Acknowledgments/Funding Sources" "texto" => "<p id="par0150" class="elsevierStylePara elsevierViewall">This study received a research grant from <span class="elsevierStyleGrantSponsor" id="gs1">Fundação para a Ciência e Tecnologia</span> (<span class="elsevierStyleGrantNumber" refid="gs1">FCT-PTDC/BIM-MEC/0650/2012</span>).</p>" "vista" => "all" ] ] ] "idiomaDefecto" => "en" "url" => "/08702551/0000003800000006/v3_201911281004/S0870255117304298/v3_201911281004/en/main.assets" "Apartado" => array:4 [ "identificador" => "29261" "tipo" => "SECCION" "en" => array:2 [ "titulo" => "Artigos Originais" "idiomaDefecto" => true ] "idiomaDefecto" => "en" ] "PDF" => "https://static.elsevier.es/multimedia/08702551/0000003800000006/v3_201911281004/S0870255117304298/v3_201911281004/en/main.pdf?idApp=UINPBA00004E&text.app=https://revportcardiol.org/" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0870255117304298?idApp=UINPBA00004E" ]
| Ano/Mês | Html | Total | |
|---|---|---|---|
| 2024 Novembro | 8 | 4 | 12 |
| 2024 Outubro | 46 | 30 | 76 |
| 2024 Setembro | 36 | 29 | 65 |
| 2024 Agosto | 45 | 24 | 69 |
| 2024 Julho | 39 | 35 | 74 |
| 2024 Junho | 43 | 32 | 75 |
| 2024 Maio | 55 | 20 | 75 |
| 2024 Abril | 55 | 37 | 92 |
| 2024 Maro | 49 | 30 | 79 |
| 2024 Fevereiro | 39 | 24 | 63 |
| 2024 Janeiro | 46 | 25 | 71 |
| 2023 Dezembro | 31 | 18 | 49 |
| 2023 Novembro | 46 | 36 | 82 |
| 2023 Outubro | 29 | 16 | 45 |
| 2023 Setembro | 31 | 18 | 49 |
| 2023 Agosto | 29 | 19 | 48 |
| 2023 Julho | 42 | 10 | 52 |
| 2023 Junho | 55 | 17 | 72 |
| 2023 Maio | 47 | 30 | 77 |
| 2023 Abril | 36 | 4 | 40 |
| 2023 Maro | 61 | 27 | 88 |
| 2023 Fevereiro | 50 | 18 | 68 |
| 2023 Janeiro | 26 | 17 | 43 |
| 2022 Dezembro | 79 | 24 | 103 |
| 2022 Novembro | 60 | 26 | 86 |
| 2022 Outubro | 65 | 29 | 94 |
| 2022 Setembro | 82 | 36 | 118 |
| 2022 Agosto | 62 | 34 | 96 |
| 2022 Julho | 51 | 42 | 93 |
| 2022 Junho | 84 | 35 | 119 |
| 2022 Maio | 81 | 28 | 109 |
| 2022 Abril | 47 | 46 | 93 |
| 2022 Maro | 55 | 43 | 98 |
| 2022 Fevereiro | 37 | 38 | 75 |
| 2022 Janeiro | 32 | 26 | 58 |
| 2021 Dezembro | 42 | 32 | 74 |
| 2021 Novembro | 47 | 34 | 81 |
| 2021 Outubro | 41 | 54 | 95 |
| 2021 Setembro | 38 | 30 | 68 |
| 2021 Agosto | 32 | 38 | 70 |
| 2021 Julho | 23 | 28 | 51 |
| 2021 Junho | 31 | 20 | 51 |
| 2021 Maio | 41 | 37 | 78 |
| 2021 Abril | 43 | 37 | 80 |
| 2021 Maro | 50 | 24 | 74 |
| 2021 Fevereiro | 49 | 17 | 66 |
| 2021 Janeiro | 39 | 18 | 57 |
| 2020 Dezembro | 30 | 18 | 48 |
| 2020 Novembro | 44 | 18 | 62 |
| 2020 Outubro | 31 | 18 | 49 |
| 2020 Setembro | 30 | 9 | 39 |
| 2020 Agosto | 33 | 13 | 46 |
| 2020 Julho | 18 | 15 | 33 |
| 2020 Junho | 19 | 23 | 42 |
| 2020 Maio | 35 | 14 | 49 |
| 2020 Abril | 39 | 9 | 48 |
| 2020 Maro | 69 | 16 | 85 |
| 2020 Fevereiro | 136 | 29 | 165 |
| 2020 Janeiro | 38 | 8 | 46 |
| 2019 Dezembro | 60 | 10 | 70 |
| 2019 Novembro | 26 | 6 | 32 |
| 2019 Outubro | 47 | 15 | 62 |
| 2019 Setembro | 155 | 38 | 193 |
| 2019 Agosto | 49 | 18 | 67 |
| 2019 Julho | 39 | 31 | 70 |
Mostrar tudo
