TY - JOUR T1 - Tempol improves lipid profile and prevents left ventricular hypertrophy in LDL receptor gene knockout (LDLr-/-) mice on a high-fat diet JO - Revista Portuguesa de Cardiologia (English edition) T2 - AU - Viana Gonçalves,Igor Cândido AU - Cerdeira,Cláudio Daniel AU - Poletti Camara,Eduardo AU - Dias Garcia,José Antônio AU - Ribeiro Pereira Lima Brigagão,Maísa AU - Bessa Veloso Silva,Roberta AU - Bitencourt dos Santos,Gérsika SN - 21742049 M3 - 10.1016/j.repce.2017.02.014 DO - 10.1016/j.repce.2017.02.014 UR - https://revportcardiol.org/en-tempol-improves-lipid-profile-prevents-articulo-S2174204917302416 AB - Introduction and ObjectiveDyslipidemia is associated with increased risk of cardiovascular disease and atherosclerosis, and hence with high morbidity and mortality. This study investigated the effects of the nitroxide 4-hydroxy-2,2,6,6-tetramethylpiperidine 1-oxyl (Tempol) on lipid profile and cardiac morphology in low-density lipoprotein (LDL) receptor gene knockout (LDLr-/-) mice. MethodsMale LDLr-/- mice (three months old, approximately 22 g weight) were divided into the following groups: controls, including (1) standard chow (SC, n=8) and (2) high-fat diet (HFD, n=8); and treatment, including (3) standard chow + Tempol (SC+T, n=8) (30 mg/kg administered by gavage, once daily) and (4) high-fat diet + Tempol (HFD+T, n=8) (30 mg/kg). After 30 days of the diet/treatment, whole blood was collected for analysis of biochemical parameters (total cholesterol, triglycerides [TG], high-density lipoprotein [HDL], LDL, and very low-density lipoprotein [VLDL]). The heart was removed through thoracotomy and histological analysis of the left ventricle was performed. ResultsA significant increase in TG, LDL, and VLDL and marked left ventricular hypertrophy (LVH) were demonstrated in the HFD group relative to the SC group (p<0.05), while Tempol treatment (HFD+T group) significantly (p<0.05) prevented increases in the levels of these lipid profile markers and attenuated LVH compared with the HFD group. ConclusionIn this study, Tempol showed potential for the prevention of events related to serious diseases of the cardiovascular system. ER -